GSM4766263: SEM BRD4 Untreated; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
BRD4
Cell type
Cell type Class
Blood
Cell type
SEM
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
Leukemia cell line (SEM)
treatment
Untreated (control for hexanediol)
cell type
Paediatric pro B-cell line derived from ALL with t(4;11)(q21;q23) translocation
chip antibody
Bethyl A301-985A
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed with PBS and fixed in 2mM DSG for 30 minutes and 1% FA for 30 minutes. Fixed Drosophila S2 cells were added at a 1:4 ratio (Dm:Hs) to allow reference normalization. Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBNext Ultra II DNA library preparation kit for Illumina